Journal: MethodsX
Article Title: Purification of murine cortex mitochondria using a modified magnetic bead isolation method ☆
doi: 10.1016/j.mex.2026.103871
Figure Lengend Snippet: Final purified mitochondrial fractions stained with BioTracker 633 Red Mitochondria Dye (middle) and TOM20 (left) show viable pure mitochondria (merged; right). Scale bar is 5 µm.
Article Snippet: Mitochondria Extraction Kit – Tissue (Miltenyi Biotec, Cat#130-097-340) Mitochondria Isolation Kit, mouse tissue (Miltenyi Biotec, Cat#130-096-946) Myelin Removal Beads II, human, mouse, rat (Miltenyi Biotec, Cat#130-096-433) Dulbecco’s PBS (1X), without Ca and Mg, without Phenol Red (Capricorn Scientific, Cat#PBS-1A) Roche cOmpleteTM, Mini, EDTA-free Protease Inhibitor Cocktail (Roche, Cat#11836170001) Bovine Serum Albumin heat shock fraction, pH 7, ≥ 98 % (Sigma-Aldrich, Cat#A7906) BioTracker 633 Red Mitochondria Dye (Sigma-Aldrich, Cat#SCT137) SuperSignalTM West Femto Maximum Sensitivity Substrate (Thermo Scientific, Cat#34096) PierceTM BCA Protein Assay Kit (Thermo Scientific, Cat#23227)
Techniques: Purification, Staining